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• Other effectors were added at estimated intracellular concentrations. ... We reported previously that when all known effectors were present in combination at physiological concentrations, the net effect was no change in RLGPa activity. ... We conclude that a combination of the known effectors of phosphorylase a activity, when present at estimated intracellular concentrations, is inhibitory. Of these effectors, only glucose changes greatly in vivo. ...
• , small-molecular-weight effectors must play a major role in regulating phosphorylase a activity in vivo (14, 45, 48, 50, 51, 67). ...
• Even though small-molecular-weight effectors are likely to play a regulatory role, we were surprised to find that, when all of the known endogenous small-molecular-weight effectors were added to a rat liver phosphorylase a preparation at estimated physiological concentrations and under simulated in vivo conditions, there was no net change in activity compared with that in the absence of effectors (13). Thus known endogenous effectors of phosphorylase a did not explain the lower-than-expected in vivo enzymic activity in rats. In the present study, we have investigated the possible role of the known effectors of phosphorylase a activity, alone or in various combinations, by use of recombinant human liver phosphorylase a. ...
• Our hypothesis was that known endogenous effectors of phosphorylase a cannot explain phosphorylase a regulation in humans or rats. ...
• For the studies of all allosteric effectors, the assay in the glycogenolytic direction was used. ...
• Therefore, intracellular concentrations of the effectors were estimated by dividing their measured concentrations in a liver extract by 0. ...
• Control (100%) is the activity in the absence of any effectors. ...
• , control activities representing 100% at concentrations of 1 and 5 mM Pi in the absence of effectors, were 1. ...
•   Estimated physiological concentrations of effectors in rat and human livers .
•   Effect on human liver glycogen phosphorylase a Km for Pi of effectors .
• Effect of combinations of effectors on phosphorylase a activity. In subsequent experiments, we determined HLGPa activity at approximate physiological concentrations and in combinations of each of the effectors. The concentrations of each effector were those typically found in rat liver intracellular water (Table 2), because we wished to compare the data with those of rat phosphorylase a and because the concentrations and possible changes under different metabolic conditions are not known for all effectors in humans. ...

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• © 2002 by Society for Leukocyte Biology Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses .

• Nuclear lipids: key signaling effectors in the nervous system and other tissues1 .

• Environmental factors as regulators and effectors of multistep carcinogenesis .

• 2196-2207, 1999 Hes1 and Hes5 as Notch effectors in mammalian neuronal differentiation .
• These results demonstrate that Hes1 and Hes5 are essential Notch effectors in regulation of mammalian neuronal differentiation. ...
• These results strongly support the conclusion that the failure of caNotch to inhibit neuronal differentiation was not due to the advancement of neuronal differentiation at the time of viral infection, but rather due to a lack of essential effectors. ...
• These results demonstrate that Hes1 and Hes5 function as essential Notch effectors in regulation of neuronal differentiation. ...
• In addition, these data demonstrated that Hes1 can substitute for the inhibition of differentiation, thus strongly supporting the conclusion that Hes genes are essential Notch effectors for inhibition of neuronal differentiation. ...

• Downstream effectors of oncogenic ras in multiple myeloma cells .

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