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• The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements .
• Immunodepletion using antibodies specific for the exosomal protein PM-Scl75 demonstrated that the human exosome complex is required for efficient 3'–5' exonucleolytic decay. ...
• Distal regions of the transcript can influence poly(A) shortening, as efficient deadenylation is mediated through an interaction between the deadenylase PARN and the 5' mRNA cap (Dehlin et al. ...
• Consistent with this hypothesis, it is interesting to note that immunodepletion of exosomal components with PM-Scl75 antiserum consistently resulted in less efficient deadenylation of RNA substrates as well (Figure 4B). ...
• The loaded exosome then may attract the PARN deadenylase to the RNA, helping to initiate efficient shortening of the poly(A) tail. ...
• (1998) Rrp6p, the yeast homologue of the human PM-Scl 100-kDa autoantigen, is essential for efficient 5. ...
• (1999) The exosome subunit Rrp43p is required for the efficient maturation of 5. ...

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