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• Electrophoresis of Chromatin on Nondenaturing Agarose Gels Containing Mg.
• We show that nondenaturing agarose gels can be used for the study of the structure and dynamic properties of native (uncross-linked) chromatin. In gels containing 1. ... This surprising behavior is not observed in low ionic strength gels. Fragments with less than 6 nucleosomes and low content of histones H1-H5 give rise to broad bands in gels with Mg. ...
• Our electrophoretic method does not require the cross-linking of the chromatin samples, but the structures produced by the association of uncross-linked oligonucleosome fragments are stable enough to form the well defined bands detected in nondenaturing gels at the end of the electrophoresis. ...
• The length of the isolated DNA corresponding to the different chromatin fragments was analyzed on agarose gels stained with ethidium bromide. The histone composition of the different samples was analyzed on SDS-polyacrylamide gels stained with the fluorescent dye Nile red(36). ...
• Electrophoresis of chromatin fragments on gels containing 0. ... 5% agarose gels instead of polyacrylamide gels. These gels were pre-electrophoresed, and the buffer was recirculated between the electrode compartments. ... 5% agarose gels (10 cm long) of higher ionic strength contained TB and the concentrations of MgCl indicated in the figure legends. ...
• Behavior of Chromatin Fragments of Different Molecular Weights in Electrophoretic Gels of Low Ionic Strength.
• 5% agarose gels containing 0. ... The relative retardation of the mononucleosome band produced by this nondenaturing agarose gel is lower than that observed in low ionic strength polyacrylamide gels(37). Nevertheless, whereas oligonucleosomes cannot enter the polyacrylamide gels(38) , the agarose gels described in this work can be used even with chromatin samples of high molecular weight. ... 1 TB agarose gels the electrophoretic mobility of native chromatin samples having a wide range of molecular weights is in all cases lower than that of the corresponding free DNA obtained from these samples.

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